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KMID : 0613820110210081176
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Journal of Life Science
2011 Volume.21 No. 8 p.1176 ~ p.1182
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Effects of Moderate Exercise Training on Splenocyte Inflammatory Cytokine Production in High Fat Diet Induced Obese Mice
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Chang Hyuk-Ki
Park Hee-Geun
Jeong Jun-Hyun
Lee Wang-Lok
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Abstract
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The present research was conducted to investigate the effect of moderate exercise training on splenocyte inflammatory cytokines production in high fat diet-induced obese mice. To determine the effects of exercise training and low fat diet on splenocyte, C57BL/6 male mice (4 weeks aged, n=20) were fed a high fat diet (45% fat diet) for 5 weeks so that obesity was achieved intentionally. These obese mice were then divided into 2 groups; HLC (low fat diet and control n=10), and HLE (low fat diet and exercise n=10). HLE mice performed 8 weeks of exercise training on a motorized treadmill by running for 30-60 min/day at 10-22 m/min, 0% grade, five times per week. After exercise training, all the splenocyte was collected and Con A (concanvalin A, 10㎍/㎖) was used to stimulate the cells, after which IL-1β, IL-6, TNF-α were measured by bio plex. Independent t-test was used and p value under 0.05 was considered a statistical significance. Splenocyte IL-1β, IL-6, TNF-α production of HLE stimulated by Con A was significantly lower than that of HLC (p＜0.01). These findings suggest that moderate exercise has beneficial effects on splenocyte inflammatory cytokines in high fat diet induced obese mice.
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KEYWORD
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Fat diet, obese, exercise training, splenocyte, inflammatory cytokines
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